rat bone marrow mesenchymal stem cells bmscs  (Procell Inc)

Journal: Frontiers in Pharmacology

Article Title: Probiotic biofilm modified bioceramics for bone defect healing via osteogenesis, angiogenesis, and immune modulation

doi: 10.3389/fphar.2025.1588023

Figure Lengend Snippet: LA@BC promoted BMSCs Osteogenic differentiation in vitro . (A) Live (green)/dead (red) staining of BMSCs after 24 h. (B) Cell proliferation of BMSCs cultured with BC, LA@BC for 24, 48 and 72 h (C) ALP staining of BMSCs after 7 days. (D) Alizarin Red S staining of BMSCs after 21 days. (E, F) Quantitative analysis of ALP staining, and Alizarin Red S staining. (G, H) Relative mRNA expression of Wnt, and β-catenin of BMSCs on day 7. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The rat bone marrow mesenchymal stem cells (BMSCs) were extracted from the bone marrow cavity of 4-week-old Sprague Dawley (SD) rats and co-cultured with α-MEM supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin solution.

Techniques: In Vitro, Staining, Cell Culture, Expressing

Journal: Chinese Medicine

Article Title: Traditional Chinese medicine Youguiyin decoction ameliorate glucocorticoid-induced osteonecrosis in rat by modulating ROS/PHD2/HIF-1α oxidative stress signaling pathway in bone marrow mesenchymal stem cells

doi: 10.1186/s13020-025-01113-1

Figure Lengend Snippet: Promoting effect of YGY medicated serum on osteogenic differentiation of dexamethasone-induced BMSCs. A Specific procedure and grouping of cell experiments. B Alizarin red staining results of each group of cells. Scale bar: 200 μm. C ROS fluorescence results for each group of cells. Scale bar: 100 μm. D Levels of oxidative stress for each group of cells (n = 6 technical replicates). E Number of calcium nodule deposits for each group of cells (n = 9 technical replicates). F Western blot results for each group of cells. G Quantitative analysis results of Western Blot (n = 3 independent experiments). Statistical analysis: Unpaired t-test. ns P > 0.05,*P < 0.05, **P < 0.01 and ***P < 0.001

Article Snippet: Rat bone marrow mesenchymal stem cells (BMSCs) were obtained from Procell Life Science and Technology Co., Ltd (Wuhan, China) and maintained in α-MEM culture medium at a 5% CO 2 concentration and 37 °C.

Techniques: Staining, Fluorescence, Western Blot

Journal: Chinese Medicine

Article Title: Traditional Chinese medicine Youguiyin decoction ameliorate glucocorticoid-induced osteonecrosis in rat by modulating ROS/PHD2/HIF-1α oxidative stress signaling pathway in bone marrow mesenchymal stem cells

doi: 10.1186/s13020-025-01113-1

Figure Lengend Snippet: PHD2 knockdown hindered the promotion of osteogenic differentiation of dexamethasone-induced BMSCs by YGY medicated serum. A Specific procedure and grouping of cell experiments. B Alizarin red staining results for each group of cells. Scale bar: 200 μm. C ROS fluorescence results for each group of cells. Scale bar: 100 μm. D Western blot analysis of PHD2 knockdown in BMSCs. E Quantitative analysis results of Western Blot of PHD2 knockdown in BMSCs (n = 3 independent experiments). F Number of calcium nodule deposits for each group of cells (n = 9 technical replicates). G Western blot results for each group of cells. H Quantitative analysis results of western blot (n = 3 independent experiments). Statistical analysis: Unpaired t-test. ns P > 0.05, ** P < 0.01 and *** P < 0.001

Article Snippet: Rat bone marrow mesenchymal stem cells (BMSCs) were obtained from Procell Life Science and Technology Co., Ltd (Wuhan, China) and maintained in α-MEM culture medium at a 5% CO 2 concentration and 37 °C.

Techniques: Knockdown, Staining, Fluorescence, Western Blot

Journal: Chinese Medicine

Article Title: Traditional Chinese medicine Youguiyin decoction ameliorate glucocorticoid-induced osteonecrosis in rat by modulating ROS/PHD2/HIF-1α oxidative stress signaling pathway in bone marrow mesenchymal stem cells

doi: 10.1186/s13020-025-01113-1

Figure Lengend Snippet: HIF-1α knockdown promoted the promotion of osteogenic differentiation of dexamethasone-induced BMSCs by YGY-medicated serum. A Specific procedure and grouping of cell experiments. B Alizarin red staining results for each group of cells. Scale bar: 200 μm. C ROS fluorescence results for each group of cells. Scale bar: 100 μm. D Western blot analysis of HIF-1α knockdown in BMSCs. E Quantitative analysis results of Western Blot of HIF-1α knockdown in BMSCs (n = 3 independent experiments). F Number of calcium nodule deposits for each group of cells (n = 9 technical replicates). G Western blot results for each group of cells. H Quantitative analysis results of western blot (n = 3 independent experiments). Statistical analysis: Unpaired t-test. ns P > 0.05, * P < 0.05, ** P < 0.01 and *** P < 0.001

Article Snippet: Rat bone marrow mesenchymal stem cells (BMSCs) were obtained from Procell Life Science and Technology Co., Ltd (Wuhan, China) and maintained in α-MEM culture medium at a 5% CO 2 concentration and 37 °C.

Techniques: Knockdown, Staining, Fluorescence, Western Blot